Heat validating system catalog the alternative dating
The cells are stimulated, if required, to induce transcription of Ren SP luciferase from the cloned promoter.
Promoter activity is then quantified using a Light Switch Luciferase Assay Kit and a luminometer to measure the amount of luminescence produced.
Active Motif also offers a HSF Pathway Reporter Stable Cell Line to facilitate high-throughput screening of the heat shock pathway.
HT1080 human fibrosarcoma cells were transfected with the promoter reporter constructs shown.
These constructs are a subset of the 18,000-member Light Switch Promoter Reporter Go Clone™ Collection that were selected based on sequence motif analysis, published information and in-house analysis of each promoter's activity* using our Light Switch Luciferase Assay.
The Heat Shock Biomarker Set contains 8 of the highest responding promoters under our chosen test conditions, as well as the promoters of 2 housekeeping genes (Figure 1).
The regulation of heat-shock genes is critical in cell stress response and the maintenance of cellular homeostasis.
Heat-shock gene transcription is regulated by HSF (heat shock factor) transcription factors in response to various types of environmental stress such as heat, starvation, hypoxia, infection and toxin exposure.
The Light Switch™ Validated Heat Shock (HSF) Pathway Collection is a set of sequence-verified, transfection-ready, promoter reporter constructs that can be used to accurately quantify transcriptional activation in response to induction of the heat shock pathway.
The collection of validated Light Switch heat shock-related promoter reporters enables you to: The Light Switch Luciferase Assay System is a complete solution for studying transcriptional & post-transcriptional gene regulation in living mammalian cells.